NGS Read Mapping & BAM Generation

FASTQ → BAM (Reference-based)

I provide a reliable NGS read mapping service, converting raw FASTQ files into analysis-ready BAM files aligned to a reference genome.

This service is designed for researchers and projects that need clean, standardized alignment outputs before downstream analyses.

Suitable for

  • Whole-Genome Sequencing (WGS)

  • Genotype-by-Sequencing (GBS)

  • Amplicon sequencing

  • Other FASTQ-based NGS datasets

What you get

  • Coordinate-sorted BAM file

  • BAM index (.bai)

  • FastQC reports (before and after trimming)

  • Basic alignment statistics (flagstat, idxstats)

All files are delivered in standard formats commonly used in genomics and bioinformatics workflows.

Scope

  • Reference-based mapping only (no de novo assembly)

  • One reference genome per project

  • Reference provided as FASTA or via NCBI / Ensembl accession

  • Variant calling and downstream analyses are not included

Data definition

  • Input: compressed FASTQ files (.fastq.gz)

  • One sample = one pair of FASTQ files (R1 + R2)

  • Data volume is calculated based on compressed FASTQ size

Projects larger than 35 samples and/or 140 GB should be discussed in advance.

Typical use cases

  • Preparing BAM files for variant calling

  • Standardizing alignments across samples or projects

  • Structuring NGS data for population genomics or other downstream analyses

Interested in this service?
Get in touch to discuss your dataset and requirements via Upwork, Fiverr or via email.